There is the problem of DNA & RNA contamination of vaccines

"We now know that genes are parts of the DNA strands in cells, which we
call chromosomes. Each organism has a specific number of chromosomes, and
furthermore has two of each type of chromosome. Humans, for example, have
46 total chromosomes, and 23 types of chromosomes. In most organisms, one
of each type of chromosome is received from each parent. '

The problem with animal cells is that during serial passage of the virus
through the animal cells, is that animal RNA and DNA can be transferred
from one host to another and undetected animal viruses may slip past
quality control testing procedures as in 1955 through 1961 with SV40 which
stands for simian #40 (meaning the 40th virus found) which has oncogenic
properties (causing cancer) What other viruses could be slipping by that we
don't know of?

Severe Acute Hepatitis B Infection After Vaccination Liver Dysfunction and
DNA Antibodies After Hepatitis B Vaccination

They are also actually using DNA to deliver vaccines -

Genetically engineered vaccines

'Inherently unpredictable and possibly dangerous'

In recent years, genetically engineered vaccine strategies have been rushed
into common use within such fields as medicine, veterinary medicine and
fish farming. Some scientists contend that such vaccines are totally
innocuous. But a recent and major research report by Professor Terje
Traavik reduces the 'safe technology' to sheer naive optimism, and warns in
conclusion that 'many live, genetically engineered vaccines are inherently
unpredictable (and) possibly dangerous.' Martin Jalleh highlights the
compelling findings of the report - which make the arguments for
genetically engineered vaccines look frail and move Traavik to call on the
scientific community to go beyond the 'Holy Grail' of medicine.

Bacterial DNA Boosts Effectiveness of Vaccines
"Scientists at the Veterinary Infectious Disease Organization in Saskatoon
have shown bacterial DNA can enhance the effectiveness vaccines used to
protect livestock from disease.

Certain segments of bacterial DNA can stimulate a danger signal in the
immune systems of animals but the animal's own DNA lacks that capability.
VIDO Bacterial DNA Research Coordinator Dr. George Mutwiri says scientists
are using that difference by including bacterial DNA in vaccines to signal
danger and stimulate the animal's immune system into action. "


"Exposure to even a minute amount of pathogen can cause the human immune
system to respond. In 1796, Edward Jenner discovered that people could
develop a greater immunity to the smallpox virus if they were inoculated
with a solution containing the similar, but less dangerous, cowpox virus.
Jonas Salk refined this process in the development of the polio vaccine,
which was made from a weakened form of the poliovirus. However, there was
danger associated with this method because occasionally these so-called
"weakened viruses" would be strong enough to cause disease.

Although the Jenner and Salk vaccines contained DNA, the vaccines that Liu
and her colleagues are developing contain only the strands of plasmid DNA
(DNA that can replicate independently of chromosomal DNA) needed to fight
disease. These custom-made vaccines can more effectively elicit a human
immune system response to pathogens such as HIV. And, unlike vaccines that
utilize weakened viruses, plasmid DNA vaccines do not pose the threat of
actually spreading the disease they are meant to fight."

GE crops contain bacterial DNA that may be hazardous to health
(Genetically engineered crops with bacterial DNA - if they are a danger,
how about vaccines with bacterial DNA?)


"Furthermore, it has been demonstrated that DNA is not broken down in the
gastro-intestinal tract to the extent formerly believed. Ingested DNA
sequences large enough to contain whole genes have remained intact and
entered the blood and tissues. "  (so what about oral polio vaccine?  )

"Gene fragments from the food are circulated and sometimes integrated into
the chromosomes of the predator. Similarly, the products of genes on the
DNA of bacteria (principally proteins but sometimes RNA) governed how the
bacterium interacted with the animal host. "

"However, it was recently discovered that the DNA sequence motifs serve as
important signals that allow animals to recognize the genes of bacterial
pathogens and turn on inflammation defenses. GM crops all contain bacterial
DNA from the plasmids used in engineering their construction. "

"DNA vaccines have generated a huge literature and clinical applications
showing the activity and cellular incorporation of DNA administered by
oral, inhalation, injection, vaginal or dermal application (Molling
1997,Donnoley et al 1997 and Gurunathan et al 2000). Ingestion of bacteria
does not appear to be an effective means of delivering DNA because the
bacterial cell walls effectively contain the nucleic acid (for example, in
yogurt the milk products are digested but the bacteria of the culture are
passed intact). Lysis genes have been found necessary and effective in
triggering release of DNA for mucosal vaccine delivery (Jani and Mekalanos
200). In contrast , the crops eaten by animals release oligonucleotides and
DNA peptide complexes during digestion and such molecules circulate to a
significant degree."

"Finally, Gorecki and Simons (1999) pointed out a danger to the fetus in
DNA vaccination of the mother. That danger was the creation of tolerance in
the fetus leading to individuals more susceptible to infection and/or they
may become carriers. The introduction of genes with bacterial CpG motif to
the fetus is likely to have untoward consequences."

"In conclusion, the bacterial genes used in GM crops have been found to
have significant impacts on the individuals ingesting GM crops. The impacts
include inflammation, arthritis and lymphoma promotion. The consequence of
GM food genes being incorporated into the chromosomes of somatic cells of
those consuming GM food and their unborn has been ignored by those charged
with evaluating the hazards of GM crops."

"Schubbert R, Hohlweg U, Renz D and Doerfler W (1998). "On the fate of
orally ingested foreign DNA in mice: chromosomal association and placental
transmission to the fetus" (1998) Mol Gen Genet 259: 569-576"

"Gorecki D and Simons J "The dangers of DNA vaccination" 1999 Nature
Medicine 5,126'

"Today, DNA vaccination is still an experimental procedure. It has been
tested on humans and proven to be harmless. However, the immunizing effect
is still too low to be of practical use. " - OH REALLY???????

****** papers_PDF/s97_cgi.pdf
] Regulation of DNA vaccines and gene therapy on animals