Induction of lupus autoantibodies
J Autoimmun. 2003
* Kuroda Y,
* Yoshida H,
* Behney KM,
* Mizutani A,
* Akaogi J,
* Nacionales DC,
* Lorenson TD,
* Rosenbauer RJ,
* Reeves WH.
Division of Rheumatology and Clinical Immunology, Department of Medicine, University of Florida, P.O. Box 100221, 1600 SW Archer Road, Gainesville, FL 32610-0221, USA. email@example.com
Exposure to the hydrocarbon oil pristane induces lupus specific autoantibodies in non-autoimmune mice. We investigated whether the capacity to induce lupus-like autoimmunity is a unique property of pristane or is shared by other adjuvant oils. Seven groups of 3-month-old female BALB/cJ mice received a single intraperitoneal injection of pristane, squalene (used in the adjuvant MF59), incomplete Freund's adjuvant (IFA), three different medicinal mineral oils, or saline, respectively.
Serum autoantibodies and peritoneal cytokine production were measured.
In addition to pristane, the mineral oil Bayol F (IFA)
and the endogenous hydrocarbon squalene both induced anti-nRNP/Sm and -Su
autoantibodies (20% and 25% of mice, respectively). All of these hydrocarbons
had prolonged effects on cytokine production by peritoneal APCs. However, high
levels of IL-6, IL-12, and TNFalpha production 2-3 months after intraperitoneal
injection appeared to be associated with the ability to induce lupus
autoantibodies.The ability to induce lupus autoantibodies is shared by several
hydrocarbons and is not unique to pristane. It correlates with stimulation of
the production of IL-12 and other cytokines, suggesting a relationship with a
hydrocarbon's adjuvanticity. The potential to induce autoimmunity may complicate
the use of oil adjuvants in human and veterinary vaccines.
PMID: 12892730 [PubMed - indexed for MEDLINE]